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Vydac® Everest™ HPLC Columns

High Resolution for Complex Peptide Digests


• Exceptionally high peak counts for better characterization
• Unique selectivity for both hydrophilic and hydrophobic peptides
• Excellent sensitivity with little or no TFA in the mobile phase

Everest™ columns have unique selectivity and sensitivity, which are the result of bonding technology that improves C18 surface coverage and deactivates residual silanols. Previously, the best 300Å C18 chemistries have had carbon coverage in the 2.8 to 3.6μmol/m2 range. Everest™ C18 coverage is in excess of 4μmol/m2 and approximates the theoretical limit based on surface area. This increased shielding of the base silica increases column life and reduces the amount of TFA required to shield the silica.

Reference Information:


Vydac® Everest™ Phase Specifications ​  

PhasePore Size (Å) ​Surface Area (m2/g)​Carbon Load (%)​End- capped Chromatographic Properties Application/Benefit USP L-code
238EV C18 ​300 ​70–110 ​6 ​Yes ​Maximum surface coverage for highest resolution of complex samples. Complex enzymatic digests (>12 proteins). L1 ​